Our research focuses on human tumor metabolism with the dual goals of therapeutic discovery and technology development. By supplying 13C-labled metabolic substrates (such as glucose, glutamine, and lactate) to living cells, we are able to then analyze isotopomer patterns of key metabolites by mass spectrometry. This data yields information on both pathway activities and metabolite pool sizes. In particular, we are able to determine activity rates of glycolysis, the pentose phosphate pathway, glutaminolysis, the TCA cycle, fatty acid biosynthesis and oxidation, and the biosynthesis of most non-essential amino acids. In addition, we have the ability to perform a wide range of complementary assays, such as large scale unbiased metabolite profiling by LC/MS, rapid substrate profiling by YSI analyzer, bioenergetic analysis (NAD(P)H and ATP/ADP/AMP) by HPLC and respiratory capacity by Seahorse XF.